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Addgene

pBABE zeo Ecotropic Receptor
(Plasmid #10687)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10687 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-zeo
  • Backbone size w/o insert (bp) 4888
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ecotropic Receptor
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2300
  • GenBank ID
    M26687
  • Entrez Gene
    Slc7a1 (a.k.a. 4831426K01Rik, AI447493, Atrc-1, Atrc1, Cat1, Rec-1, Rev-1, mCAT-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expression of the gene encoding for the mouse ecotropic retroviral receptor increases the susceptibility of cells to infection with retroviral vectors of ecotropic host range.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE zeo Ecotropic Receptor was a gift from William Hahn (Addgene plasmid # 10687 ; http://n2t.net/addgene:10687 ; RRID:Addgene_10687)
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