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AE-Polyubiquitin-GCaMP6s
(Plasmid #106868)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac
  • Backbone size w/o insert (bp) 5246
  • Total vector size (bp) 6599
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AE-Polyubiquitin-GCAaMP6s
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1353
  • Promoter Ae. aegypti polyubiquitin (PUb)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGACGGTCACGGCGGGCATGTCGACGCGGCCGCTATCTTTACATGTAGCTTGTGCATTGA
  • 3′ sequencing primer AGCCATACCATGATGATGATGATGATGAGAACCCATCTCGAGATTCGTTGAAATCTCTGT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AE-Polyubiquitin-GCaMP6s was a gift from Omar Akbari (Addgene plasmid # 106868 ; http://n2t.net/addgene:106868 ; RRID:Addgene_106868)

  • For your References section:

    Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies. Bui M, Shyong J, Lutz EK, Yang T, Li M, Truong K, Arvidson R, Buchman A, Riffell JA, Akbari OS. BMC Neurosci. 2019 Jun 17;20(1):27. doi: 10.1186/s12868-019-0511-y. 10.1186/s12868-019-0511-y PubMed 31208328
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