pMKO.1 GFP B55 alpha shRNA 3-1
(Plasmid #10679)

• Depositing Lab: William Hahn
• Publication: Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Backbone

• Vector backbone: pMKO.1
• Backbone size w/o insert (bp): 6700
• Vector type: Mammalian Expression, Retroviral, RNAi
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PP2A B55 alpha shRNA
• Alt name: PP2A B55 alpha
• Alt name: B55 alpha
• Species: H. sapiens (human)
• Insert Size (bp): 58
• Entrez Gene: PPP2R2A (a.k.a. B55A, B55ALPHA, PR52A, PR55A, PR55alpha)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLXSN 5'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To make retroviruses expressing shRNAs, oligonucleotides were inserted in the vector pMKO.1-puro modified by replacing the puromycin resistance gene with hrGFP (pMKO.1-GFP). The shRNAs were generated by cloning nucleotides 1156-1175 of PP2A B55 alpha followed by a 6 bp loop and the corresponding antisense sequence followed by five thymidines into pMKO.1-GFP.