pBabe HcRed Ras
(Plasmid #10678)

• Depositing Lab: William Hahn
• Publication: Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74.

Backbone

• Vector backbone: pBabe HcRed
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: HcRed

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hras V12
• Alt name: v-Ha-ras
• Alt name: Hras
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Mutation: G12V, constitutively active
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (not destroyed)
• 3′ cloning site: SnaBI (not destroyed)
• 5′ sequencing primer: pBabe5

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBabe-puro-Ras was digested with ClaI, blunted and purified. Linearized vector was digested with HindIII, releasing the 5.6kb vector piece. pcDNA-HcRed was digested with HindIII and PmeI, and the 700bp piece containing HcRed was ligated into pBabe.