-
Purpose(Empty Backbone)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10677 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBABE HcRed
- Backbone size (bp) 5100
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersHcRed
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE-3 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pBabe-puro was digested with ClaI, blunted and purified. Linearized vector was digested with HindIII, releasing the 4.4kb vector piece. pcDNA-HcRed was digested with HindIII and PmeI, and the 700bp piece containing HcRed was ligated into pBabe.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBabe HcRed was a gift from William Hahn (Addgene plasmid # 10677 ; http://n2t.net/addgene:10677 ; RRID:Addgene_10677) -
For your References section:
Transformation of human and murine fibroblasts without viral oncoproteins. Boehm JS, Hession MT, Bulmer SE, Hahn WC. Mol Cell Biol. 2005 Aug . 25(15):6464-74. 10.1128/MCB.25.15.6464-6474.2005 PubMed 16024784
Map uploaded by the depositor.
