pBABE GFP
(Plasmid #10668)

• Purpose: (Empty Backbone) Empty backbone for retroviral gene expression. Select recipient cells using GFP.
• Depositing Lab: William Hahn
• Publication: Viral vectors. (unpublished)

Backbone

• Vector backbone: pBABE
• Backbone size (bp): 5000
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE-3

Resource Information

• Articles Citing this Plasmid:
  • 20 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Replaced the puromycin resistance gene from pBABE-puro with GFP for cell sorting. HindIII-ClaI GFP ORF from phrGFP-c (Stratagene) was cloned into pBABE-puro. hrGFP =humanized Renilla green fluorescent protein.

Information for Cloning Grade DNA (Catalog # 10668-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here