pKP112 (pRS406-GFP-ADH1t)
(Plasmid
#106472)
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Purpose(Empty Backbone) Vector for creating C-terminal GFP fusion proteins in yeast.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 106472 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS406
- Backbone size (bp) 5306
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Vector typeBacterial Expression, Yeast Expression
- Promoter None
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Selectable markersURA3
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKP112 (pRS406-GFP-ADH1t) was a gift from Daniel Klionsky (Addgene plasmid # 106472 ; http://n2t.net/addgene:106472 ; RRID:Addgene_106472) -
For your References section:
A newly characterized vacuolar serine carboxypeptidase, Atg42/Ybr139w, is required for normal vacuole function and the terminal steps of autophagy in the yeast Saccharomyces cerevisiae. Parzych KR, Ariosa A, Mari M, Klionsky DJ. Mol Biol Cell. 2018 May 1;29(9):1089-1099. doi: 10.1091/mbc.E17-08-0516. Epub 2018 Mar 22. 10.1091/mbc.E17-08-0516 PubMed 29514932
