pKP136 (pRS406-PRC1p-PRC1-PA-ADH1t)
(Plasmid #106471)

• Purpose: Expresses Prc1 with a C-terminal PA tag in yeast cells
• Depositing Lab: Daniel Klionsky
• Publication: Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-1099. doi: 10.1091/mbc.E17-08-0516. Epub 2018 Mar 22.

Backbone

• Vector backbone: pRS406
• Total vector size (bp): 7353
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PRC1
• Alt name: CPY
• Alt name: Carboxypeptidase Y
• Alt name: Proteinase C
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1596
• Promoter: PRC1
• Tag / Fusion Protein:
  • PA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (unknown if destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: M13 REV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry