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pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]
(Plasmid #106469)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106469 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKP112
  • Total vector size (bp) 7678
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YBR139W(S219,D415,H474A)
  • Alt name
    ATG42
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1524
  • Mutation
    Changed Serine 219, Aspartate 415 and Histidine 474 to Alanine
  • Promoter YBR139W
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer M13 REV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t] was a gift from Daniel Klionsky (Addgene plasmid # 106469 ; http://n2t.net/addgene:106469 ; RRID:Addgene_106469)

  • For your References section:

    A newly characterized vacuolar serine carboxypeptidase, Atg42/Ybr139w, is required for normal vacuole function and the terminal steps of autophagy in the yeast Saccharomyces cerevisiae. Parzych KR, Ariosa A, Mari M, Klionsky DJ. Mol Biol Cell. 2018 May 1;29(9):1089-1099. doi: 10.1091/mbc.E17-08-0516. Epub 2018 Mar 22. 10.1091/mbc.E17-08-0516 PubMed 29514932
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