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Addgene

pCR3.1-mutLuc
(Plasmid #106458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR3.1
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    luciferase reporter, CMV promoter (3 NFkB sites mutated)
  • Species
    Synthetic
  • Mutation
    3 NFkB sites mutated

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR3.1-mutLuc was a gift from Georg Weber (Addgene plasmid # 106458 ; http://n2t.net/addgene:106458 ; RRID:Addgene_106458)

  • For your References section:

    Synergistic activation of the CMV promoter by NF-kappaB P50 and PKG. He B, Weber GF. Biochem Biophys Res Commun. 2004 Aug 13;321(1):13-20. doi: 10.1016/j.bbrc.2004.06.101. 10.1016/j.bbrc.2004.06.101 PubMed 15358208
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