pRSV-p65T305A,S276A
(Plasmid
#106456)
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PurposeNF-kB p65, kinase sites 276,305 mutated
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 106456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNF-kB p65, kinase sites 276,305 mutated
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SpeciesH. sapiens (human)
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Mutationkinase sites inactivated
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Entrez GeneRELA (a.k.a. AIF3BL3, CMCU, NFKB3, p65)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSV-p65T305A,S276A was a gift from Georg Weber (Addgene plasmid # 106456 ; http://n2t.net/addgene:106456 ; RRID:Addgene_106456) -
For your References section:
Phosphorylation of NF-kappaB proteins by cyclic GMP-dependent kinase. A noncanonical pathway to NF-kappaB activation. He B, Weber GF. Eur J Biochem. 2003 May . 270(10):2174-85. 10.1046/j.1432-1033.2003.03574.x PubMed 12752437