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Addgene

pEGF/HisB-cGK1
(Plasmid #106451)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106451 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGF/HisB
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyclic GMP-dependent kinase
  • Species
    M. musculus (mouse)
  • Mutation
    WT
  • Entrez Gene
    Prkg1 (a.k.a. CGKI, Gm19690, Prkg1b, Prkgr1b)
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGF/HisB-cGK1 was a gift from Georg Weber (Addgene plasmid # 106451 ; http://n2t.net/addgene:106451 ; RRID:Addgene_106451)

  • For your References section:

    Synergistic activation of the CMV promoter by NF-kappaB P50 and PKG. He B, Weber GF. Biochem Biophys Res Commun. 2004 Aug 13;321(1):13-20. doi: 10.1016/j.bbrc.2004.06.101. 10.1016/j.bbrc.2004.06.101 PubMed 15358208
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