AChE-pcDNA
(Plasmid
#106442)
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PurposeMammalian expression of murine acetylcholinesterase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 106442 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameacetylcholinesterase
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SpeciesH. sapiens (human)
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MutationWT
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Entrez GeneACHE (a.k.a. ACEE, ARACHE, N-ACHE, YT)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AChE-pcDNA was a gift from Georg Weber (Addgene plasmid # 106442 ; http://n2t.net/addgene:106442 ; RRID:Addgene_106442) -
For your References section:
Acetylcholinesterase supports anchorage independence in colon cancer. Syed M, Fenoglio-Preiser C, Skau KA, Weber GF. Clin Exp Metastasis. 2008;25(7):787-98. doi: 10.1007/s10585-008-9192-0. Epub 2008 Jul 9. 10.1007/s10585-008-9192-0 PubMed 18612832