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Addgene

SunTag-FWAgRNA-4-22aa-TET1cd
(Plasmid #106435)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106435 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMOA34
  • Vector type
    Plant Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
  • Alt name
    SunTagFWAg4-22aa-TET1cd
  • Alt name
    gRNA: acggaaagatgtatgggctt
  • Species
    H. sapiens (human), A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    17065

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Portions of the SunTagFWAg4-22aa-TET1cd were amplified from Addgene plasmid # 60903 and # 60904, gifts from Ron Vale. The pMOA34 backbone was a gift from Zachary Nimchuk.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SunTag-FWAgRNA-4-22aa-TET1cd was a gift from Steven Jacobsen (Addgene plasmid # 106435 ; http://n2t.net/addgene:106435 ; RRID:Addgene_106435)

  • For your References section:

    Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain. Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo HY, Zhao JM, Segal DJ, Jacobsen SE. Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 10.1073/pnas.1716945115. Epub 2018 Feb 14. 10.1073/pnas.1716945115 PubMed 29444862
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