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pUBQ10::ZF1CACTA1_3xFLAG_TET1cd
(Plasmid #106434)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106434 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMDC123
  • Vector type
    Plant Expression, Synthetic Biology
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TET1 tet methylcytosine dioxygenase 1
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    5676
  • Entrez Gene
    TET1 (a.k.a. CXXC6, LCX, bA119F7.1)
  • Tag / Fusion Protein
    • 3xFLAG

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The catalytic domain of the TET1 protein (TET1cd) was amplified from the plasmid pJFA334E9, a gift from Keith Joung (Addgene plasmid # 49237)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUBQ10::ZF1CACTA1_3xFLAG_TET1cd was a gift from Steven Jacobsen (Addgene plasmid # 106434 ; http://n2t.net/addgene:106434 ; RRID:Addgene_106434)

  • For your References section:

    Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain. Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo HY, Zhao JM, Segal DJ, Jacobsen SE. Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 10.1073/pnas.1716945115. Epub 2018 Feb 14. 10.1073/pnas.1716945115 PubMed 29444862
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