pUBQ10::ZF2CACTA1_3xFLAG_TET1cd
(Plasmid #106433)

• Purpose: pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis
• Depositing Lab: Steven Jacobsen
• Publication: Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 10.1073/pnas.1716945115. Epub 2018 Feb 14.

Backbone

• Vector backbone: pMDC123
• Vector type: Plant Expression, Synthetic Biology
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pUBQ10::ZF1CACTA1_3xFLAG_TET1cd
• Species: H. sapiens (human), A. thaliana (mustard weed), Synthetic
• Insert Size (bp): 5676
• Entrez Gene: TET1 (a.k.a. CXXC6, LCX, bA119F7.1)
• Tag / Fusion Protein:
  • 3xFLAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The catalytic domain of the TET1 protein (TET1cd) was amplified from the plasmid pJFA334E9, a gift from Keith Joung (Addgene plasmid # 49237)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry