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Addgene

hASXL(1-115)-luciferase
(Plasmid #106427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-luciferase
  • Backbone manufacturer
    Addgene #18964
  • Backbone size w/o insert (bp) 7041
  • Total vector size (bp) 7400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    addition sex combs like 1 ASXL1
  • Alt name
    ASXL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    350
  • Mutation
    amino acids 1-115 fused to luciferase
  • Entrez Gene
    ASXL1 (a.k.a. BOPS, MDS)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMF-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hASXL(1-115)-luciferase was a gift from Douglas Feldman (Addgene plasmid # 106427 ; http://n2t.net/addgene:106427 ; RRID:Addgene_106427)

  • For your References section:

    An Adversarial DNA N(6)-Methyladenine-Sensor Network Preserves Polycomb Silencing. Kweon SM, Chen Y, Moon E, Kvederaviciute K, Klimasauskas S, Feldman DE. Mol Cell. 2019 Apr 5. pii: S1097-2765(19)30222-9. doi: 10.1016/j.molcel.2019.03.018. 10.1016/j.molcel.2019.03.018 PubMed 30982744
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