pCMV-H-RAN-RFP6
(Plasmid
#106426)
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PurposeH-RAN-RFP6 (RANbody RFP6::Sm-HAtag)
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 106426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Backbone manufacturerTakara/Clontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH-RAN-RFP6
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Alt nameRANbody RFP6::SmHAtag
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SpeciesSynthetic
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- His (C terminal on insert)
- Multiple HA
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
It contains a sequence from Addgene #59759.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-H-RAN-RFP6 was a gift from Joshua Sanes (Addgene plasmid # 106426 ; http://n2t.net/addgene:106426 ; RRID:Addgene_106426) -
For your References section:
Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR. Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1722491115. doi: 10.1073/pnas.1722491115. 10.1073/pnas.1722491115 PubMed 29440485