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Tol2-ngn1-Trpa1b-2A-mCherry
(Plasmid #106425)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDestTol2pA2
  • Vector type
    gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    cmlc2 promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3132

Gene/Insert 2

  • Gene/Insert name
    Trpa1b
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    4098
  • GenBank ID
    EU826642.1
  • Entrez Gene
    trpa1b (a.k.a. trpa1l, trpa2)
  • Tag / Fusion Protein
    • 2A-mCherry (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original Trpa1b cDNA plasmid is a generous gift from Dr. David Prober.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-ngn1-Trpa1b-2A-mCherry was a gift from Randall Peterson (Addgene plasmid # 106425 ; http://n2t.net/addgene:106425 ; RRID:Addgene_106425)

  • For your References section:

    A high-conductance chemo-optogenetic system based on the vertebrate channel Trpa1b. Lam PY, Mendu SK, Mills RW, Zheng B, Padilla H, Milan DJ, Desai BN, Peterson RT. Sci Rep. 2017 Sep 19;7(1):11839. doi: 10.1038/s41598-017-11791-z. 10.1038/s41598-017-11791-z [pii] PubMed 28928472
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