pCMV-Y-RAN-GFP26
(Plasmid
#106423)
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PurposeY-RAN-GFP26 (RANbody GFP26::Chicken IgY)
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 106423 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Backbone manufacturerTakara/Clontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameY-RAN-GFP26
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Alt nameRANbody GFP26::Chicken IgY
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SpeciesG. gallus (chicken), Synthetic
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- His (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-Y-RAN-GFP26 was a gift from Joshua Sanes (Addgene plasmid # 106423 ; http://n2t.net/addgene:106423 ; RRID:Addgene_106423) -
For your References section:
Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR. Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1722491115. doi: 10.1073/pnas.1722491115. 10.1073/pnas.1722491115 PubMed 29440485