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Addgene

pCMV-H-RAN-GFP1
(Plasmid #106418)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106418 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone manufacturer
    Takara/Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H-RAN-GFP1
  • Alt name
    RANbody GFP1::SmHAtag
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • Multiple HA
    • His (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

It contains a sequence from Addgene #59759.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-H-RAN-GFP1 was a gift from Joshua Sanes (Addgene plasmid # 106418 ; http://n2t.net/addgene:106418 ; RRID:Addgene_106418)

  • For your References section:

    Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR. Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1722491115. doi: 10.1073/pnas.1722491115. 10.1073/pnas.1722491115 PubMed 29440485
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