pQZ083
(Plasmid
#106417)
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PurposeExpress Complexin-1 fragment (1-83) in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 106417 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-6P-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecomplexin-1 fragment 1-83
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Alt namecomplexin-1
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SpeciesR. norvegicus (rat), Synthetic
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MutationFragment 1-83, Codon optimization
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Entrez GeneCplx1
- Promoter T7
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Tag
/ Fusion Protein
- GST tag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pGEX-5
- 3′ sequencing primer pGEX-3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQZ083 was a gift from Axel Brunger (Addgene plasmid # 106417 ; http://n2t.net/addgene:106417 ; RRID:Addgene_106417) -
For your References section:
The primed SNARE-complexin-synaptotagmin complex for neuronal exocytosis. Zhou Q, Zhou P, Wang AL, Wu D, Zhao M, Sudhof TC, Brunger AT. Nature. 2017 Aug 24;548(7668):420-425. doi: 10.1038/nature23484. Epub 2017 Aug 16. 10.1038/nature23484 PubMed 28813412
