pDC183
(Plasmid #106415)

• Purpose: Co-expresses trans SNARE complex with pQZ210 in E. coli
• Depositing Lab: Axel Brunger
• Publication: Zhou et al Nature. 2017 Aug 24;548(7668):420-425. doi: 10.1038/nature23484. Epub 2017 Aug 16.

Backbone

• Vector backbone: pETDuet-1
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: SNAP25 fragment 141-204
• Alt name: SNAP25
• Species: R. norvegicus (rat), Synthetic
• Mutation: Fragment 141-204, Codon optimization
• Entrez Gene: Snap25 (a.k.a. SNAP-25B, SNAP-25a)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pET Upstream Primer #69214-3
• 3′ sequencing primer: DuetDOWN1 Primer #71179-3

Gene/Insert 2

• Gene/Insert name: SNAP25 Fragment 7-83
• Alt name: SNAP25
• Species: R. norvegicus (rat), Synthetic
• Mutation: Fragment 7-83, Codon optimization
• GenBank ID: P60880
• Entrez Gene: Snap25 (a.k.a. SNAP-25B, SNAP-25a)
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: DuetUP2 Primer #71180-3
• 3′ sequencing primer: T7 Terminator Primer #69337-3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry