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pQZ210
(Plasmid #106414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYCDuet-1
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Rat Synaptobrevin-2 fragment 28-66
  • Alt name
    VAMP2
  • Species
    Synthetic
  • Mutation
    Fragment 28-66, Codon optimization
  • Entrez Gene
    Vamp2 (a.k.a. RATVAMPB, RATVAMPIR, SYB, Syb2)
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACYCDuetUP1 Primer #71178-3
  • 3′ sequencing primer DuetDOWN1 Primer #71179-3
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Syntaxin-1A fragment 191-256
  • Alt name
    Syntaxin-1A
  • Species
    R. norvegicus (rat), Synthetic
  • Mutation
    Fragment 191-256 , Codon optimization
  • Entrez Gene
    Stx1a
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer DuetUP2 Primer #71180-3
  • 3′ sequencing primer T7 Terminator Primer #69337-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQZ210 was a gift from Axel Brunger (Addgene plasmid # 106414 ; http://n2t.net/addgene:106414 ; RRID:Addgene_106414)

  • For your References section:

    The primed SNARE-complexin-synaptotagmin complex for neuronal exocytosis. Zhou Q, Zhou P, Wang AL, Wu D, Zhao M, Sudhof TC, Brunger AT. Nature. 2017 Aug 24;548(7668):420-425. doi: 10.1038/nature23484. Epub 2017 Aug 16. 10.1038/nature23484 PubMed 28813412
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