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Addgene

pGL3-U6-sgRNA-PGK-puromycin-AflII
(Plasmid #106404)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106404 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-U6-sgRNA-PGK-puromycin (Addgene: 51133)
  • Backbone size (bp) 4952
  • Modifications to backbone
    The MCS from Addgene 41824 containing an AflII site was introduced to facilitate Gibson assembly cloning of gRNA target sequences. In this cloning process, an EcoRI site in the original backbone was destroyed.
  • Vector type
    Mammalian Expression
  • Promoter U6
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-U6-sgRNA-PGK-puromycin-AflII was a gift from Jason Gertz (Addgene plasmid # 106404 ; http://n2t.net/addgene:106404 ; RRID:Addgene_106404)

  • For your References section:

    Multiplex Enhancer Interference Reveals Collaborative Control of Gene Regulation by Estrogen Receptor alpha-Bound Enhancers. Carleton JB, Berrett KC, Gertz J. Cell Syst. 2017 Oct 25;5(4):333-344.e5. doi: 10.1016/j.cels.2017.08.011. Epub 2017 Sep 27. 10.1016/j.cels.2017.08.011 PubMed 28964699
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