pSEVA-gRic6T
(Plasmid
#106401)
-
PurposeE.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 106401 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepSEVA644 pTargetF
- Backbone size w/o insert (bp) 4500
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Gentamicin, 10 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namesgRNA
-
SpeciesSynthetic
-
Insert Size (bp)96
- Promoter pj23119
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSEVA-gRic6T was a gift from Sheng Yang (Addgene plasmid # 106401 ; http://n2t.net/addgene:106401 ; RRID:Addgene_106401) -
For your References section:
Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system. Sun J, Wang Q, Jiang Y, Wen Z, Yang L, Wu J, Yang S. Microb Cell Fact. 2018 Mar 13;17(1):41. doi: 10.1186/s12934-018-0887-x. 10.1186/s12934-018-0887-x PubMed 29534717
