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Addgene

pCas9
(Plasmid #106396)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106396 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSEVA224
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    cas9
  • Species
    Streptococcus pyogenes
  • Promoter J23105

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Lambda red genes
  • Species
    Lambda bacteriophage
  • Promoter araBAD

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that some discrepancies were found between Addgene's quality control and the depositor's genbank sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas9 was a gift from Brian Pfleger (Addgene plasmid # 106396)

  • For your References section:

    Genetic tools for reliable gene expression and recombineering in Pseudomonas putida. Cook TB, Rand JM, Nurani W, Courtney DK, Liu SA, Pfleger BF. J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5. 10.1007/s10295-017-2001-5 [pii] PubMed 29299733
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