pBBR2k-GFPuv
(Plasmid
#106383)
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PurposeAnhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 origin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 106383 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSEVA234
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFPuv
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SpeciesSynthetic
- Promoter tet
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBBR2k-GFPuv was a gift from Brian Pfleger (Addgene plasmid # 106383 ; http://n2t.net/addgene:106383 ; RRID:Addgene_106383) -
For your References section:
Genetic tools for reliable gene expression and recombineering in Pseudomonas putida. Cook TB, Rand JM, Nurani W, Courtney DK, Liu SA, Pfleger BF. J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5. 10.1007/s10295-017-2001-5 [pii] PubMed 29299733