pMOTag-M9-4H
(Plasmid #106379)

• Purpose: Can be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulation
• Depositing Lab: Roberto Docampo
• Publication: Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi: 10.1111/jeu.12493.

Backbone

• Vector backbone: pMOTag4H
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GlmS with M9 mutation

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: CACTCATTAGGCACC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The pMOTag4H vector was a gift from Dr. Thomas Seebeck (University of Bern, Bern, Switzerland). Wild type and M9 mutated Bacillus subtilis glmS ribozymes were gifts from Dr. Vasant Muralidharan (University of Georgia). The glmS fragment with M9 mutation was cloned into pmotag4H plasmid by Teresa Cruz-Bustos.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA