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Addgene

pRV-GN
(Plasmid #106373)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106373 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-based, pSEN
  • Backbone manufacturer
    homemade, PMID: 17449199
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eGFP and geneticin/G418 resistance gene
  • Alt name
    enhanced GFP
  • Alt name
    G418

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRV-GN was a gift from Tong-Chuan He (Addgene plasmid # 106373 ; http://n2t.net/addgene:106373 ; RRID:Addgene_106373)

  • For your References section:

    Characterization of retroviral infectivity and superinfection resistance during retrovirus-mediated transduction of mammalian cells. Liao J, Wei Q, Fan J, Zou Y, Song D, Liu J, Liu F, Ma C, Hu X, Li L, Yu Y, Qu X, Chen L, Yu X, Zhang Z, Zhao C, Zeng Z, Zhang R, Yan S, Wu T, Wu X, Shu Y, Lei J, Li Y, Zhang W, Wang J, Reid RR, Lee MJ, Huang W, Wolf JM, He TC, Wang J. Gene Ther. 2017 Jun;24(6):333-341. doi: 10.1038/gt.2017.24. Epub 2017 May 4. 10.1038/gt.2017.24 PubMed 28387759
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