pSO7
(Plasmid
#106365)
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PurposeTo insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the epidermis.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 106365 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSO5
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namettTi4348_ SEC _pcol-19_OspF_3'unc54UTR
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SpeciesShigella flexneri
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GenBank IDYP_006960239
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains ttTi4348_ SEC _pcol-19_OspF_3'unc54UTR . Insertion and selection = Chr I ttTi4348 + SEC
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSO7 was a gift from Jonathan Ewbank (Addgene plasmid # 106365 ; http://n2t.net/addgene:106365 ; RRID:Addgene_106365) -
For your References section:
Modulatory upregulation of an insulin peptide gene by different pathogens in C. elegans. Lee SH, Omi S, Thakur N, Taffoni C, Belougne J, Engelmann I, Ewbank JJ, Pujol N. Virulence. 2018 Dec 31;9(1):648-658. doi: 10.1080/21505594.2018.1433969. 10.1080/21505594.2018.1433969 PubMed 29405821