pSO5
(Plasmid
#106364)
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Purpose(Empty Backbone) A vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr I.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 106364 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNP154
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains ttTi4348_ SEC _ccdB_mkate2 x 2_ccdb . Insertion and selection = Chr I ttTi4348 + SEC
The depositor notes that there are mutations in the first ccdB cassette (which may or may not render it non-functional); however, selection should still work since the second ccdB is intact.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSO5 was a gift from Jonathan Ewbank (Addgene plasmid # 106364 ; http://n2t.net/addgene:106364 ; RRID:Addgene_106364) -
For your References section:
Modulatory upregulation of an insulin peptide gene by different pathogens in C. elegans. Lee SH, Omi S, Thakur N, Taffoni C, Belougne J, Engelmann I, Ewbank JJ, Pujol N. Virulence. 2018 Dec 31;9(1):648-658. doi: 10.1080/21505594.2018.1433969. 10.1080/21505594.2018.1433969 PubMed 29405821
