pNP172
(Plasmid
#106363)
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PurposeTo insert a single copy of a construct at a site on Chr II, corresponding to mKate expressed specifically in the epidermis; can be used for N- or C-terminal fusion.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 106363 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNP154
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namettTi5605_SEC_col-12p_mkate2 x _3'tbb-2
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains ttTi5605_SEC_col-12p_mkate2 x _3'tbb-2. Insertion and selection = Chr II ttTi5605 +SEC
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNP172 was a gift from Jonathan Ewbank (Addgene plasmid # 106363 ; http://n2t.net/addgene:106363 ; RRID:Addgene_106363)
