pNP159
(Plasmid
#106361)
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PurposeTo insert a single copy of a construct at a site on Chr II, corresponding to GFP-labelled RAB-11 expressed specifically in the epidermis.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 106361 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNP154
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namettTi5605_SEC_dpy-7p_GFP_RAB-11_3'unc-54
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains ttTi5605_SEC_dpy-7p_GFP_RAB-11_3'unc-54. Insertion and selection = Chr II ttTi5605 +SEC
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNP159 was a gift from Jonathan Ewbank (Addgene plasmid # 106361 ; http://n2t.net/addgene:106361 ; RRID:Addgene_106361) -
For your References section:
Microtubule plus-end dynamics link wound repair to the innate immune response. Taffoni C, Omi S, Huber C, Mailfert S, Fallet M, Rupprecht JF, Ewbank JJ, Pujol N. Elife. 2020 Jan 29;9. pii: 45047. doi: 10.7554/eLife.45047. 10.7554/eLife.45047 PubMed 31995031