Skip to main content
Addgene

pNP154
(Plasmid #106360)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106360 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAP066
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid contains ttTi5605_SEC_ccdB_mkate2 x 2_ccdb . Insertion and selection = Chr II ttTi5605 +SEC

The depositor notes that there are mutations in the first ccdB cassette (which may or may not render it non-functional); however, selection should still work since the second ccdB is intact.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNP154 was a gift from Jonathan Ewbank (Addgene plasmid # 106360 ; http://n2t.net/addgene:106360 ; RRID:Addgene_106360)
  • For your References section:

    Microtubule plus-end dynamics link wound repair to the innate immune response. Taffoni C, Omi S, Huber C, Mailfert S, Fallet M, Rupprecht JF, Ewbank JJ, Pujol N. Elife. 2020 Jan 29;9. pii: 45047. doi: 10.7554/eLife.45047. 10.7554/eLife.45047 PubMed 31995031