pNP154
(Plasmid #106360)

• Purpose: (Empty Backbone) A vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr II.
• Depositing Lab: Jonathan Ewbank
• Publication: Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 10.7554/eLife.45047.

Backbone

• Vector backbone: pAP066
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid contains ttTi5605_SEC_ccdB_mkate2 x 2_ccdb . Insertion and selection = Chr II ttTi5605 +SEC

The depositor notes that there are mutations in the first ccdB cassette (which may or may not render it non-functional); however, selection should still work since the second ccdB is intact.