pCW57.1-MBD3L2
(Plasmid #106332)

• Purpose: All-in-one lentivector with tet-inducible MBD3L2
• Depositing Lab: Stephen Tapscott
• Publication: Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 10.7554/eLife.31023.

Backbone

• Vector backbone: pCW57.1
• Backbone size w/o insert (bp): 7792
• Total vector size (bp): 8419
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: methyl-CpG binding domain protein 3 like 2
• Alt name: MBD3L2
• Species: H. sapiens (human)
• Insert Size (bp): 627
• Entrez Gene: MBD3L2
• Promoter: tight TRE promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: tcgcctggagaattggc
• 3′ sequencing primer: agggctgccttggaaaag

Resource Information

• Supplemental Documents:
  • pCW57.1_MBD3L2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry