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Addgene

pCR3.1-OPNc M5
(Plasmid #106325)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106325 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OPNc
  • Species
    H. sapiens (human)
  • Mutation
    OPNc splice site insertion mutant EEKQNA-->EEKQANA
  • Entrez Gene
    SPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR3.1-OPNc M5 was a gift from Georg Weber (Addgene plasmid # 106325 ; http://n2t.net/addgene:106325 ; RRID:Addgene_106325)

  • For your References section:

    The osteopontin-c splice junction is important for anchorage-independent growth. Shen H, Weber GF. Mol Carcinog. 2014 Jun;53(6):480-7. doi: 10.1002/mc.21997. Epub 2013 Jan 28. 10.1002/mc.21997 PubMed 23359517
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