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PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B
(Plasmid #106309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone manufacturer
    synthetic
  • Total vector size (bp) 10200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    6xflag-RT3-3xHaloTag(MtoL)-H2B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3276
  • GenBank ID
    AF531285.1
  • Entrez Gene
    H2BC3 (a.k.a. H2B.1, H2B/f, H2BFF, HIST1H2BB)
  • Promoter EF1
  • Tag / Fusion Protein
    • 6xflag-3xHaloTag(MtoL) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer 5'-CCTACCTAGACTCAGC-3'
  • 3′ sequencing primer 5'-AAGACGGCAATATGGTGGAAAA-3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B was a gift from James Zhe Liu (Addgene plasmid # 106309 ; http://n2t.net/addgene:106309 ; RRID:Addgene_106309)

  • For your References section:

    Visualizing long-term single-molecule dynamics in vivo by stochastic protein labeling. Liu H, Dong P, Ioannou MS, Li L, Shea J, Pasolli HA, Grimm JB, Rivlin PK, Lavis LD, Koyama M, Liu Z. Proc Natl Acad Sci U S A. 2017 Dec 28. pii: 1713895115. doi: 10.1073/pnas.1713895115. 10.1073/pnas.1713895115 PubMed 29284749
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