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PurposeThe plasmid encodes the scFv against GCN4 peptide fused with HaloTag and a NLS for SunTag imaging
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 106303 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namescFv-GCN4-HaloTag-GB1-NLS
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Insert Size (bp)2031
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid cloning was based on #60906 "pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE" from Ron Vale Lab, with the replacement of sfGFP with HaloTag.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR-scFv-GCN4-HaloTag-GB1-NLS-dWPRE was a gift from James Zhe Liu (Addgene plasmid # 106303 ; http://n2t.net/addgene:106303 ; RRID:Addgene_106303) -
For your References section:
Visualizing long-term single-molecule dynamics in vivo by stochastic protein labeling. Liu H, Dong P, Ioannou MS, Li L, Shea J, Pasolli HA, Grimm JB, Rivlin PK, Lavis LD, Koyama M, Liu Z. Proc Natl Acad Sci U S A. 2017 Dec 28. pii: 1713895115. doi: 10.1073/pnas.1713895115. 10.1073/pnas.1713895115 PubMed 29284749