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Addgene

pHR-scFv-GCN4-HaloTag-GB1-NLS-dWPRE
(Plasmid #106303)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106303 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    scFv-GCN4-HaloTag-GB1-NLS
  • Insert Size (bp)
    2031

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid cloning was based on #60906 "pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE" from Ron Vale Lab, with the replacement of sfGFP with HaloTag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-scFv-GCN4-HaloTag-GB1-NLS-dWPRE was a gift from James Zhe Liu (Addgene plasmid # 106303 ; http://n2t.net/addgene:106303 ; RRID:Addgene_106303)
  • For your References section:

    Visualizing long-term single-molecule dynamics in vivo by stochastic protein labeling. Liu H, Dong P, Ioannou MS, Li L, Shea J, Pasolli HA, Grimm JB, Rivlin PK, Lavis LD, Koyama M, Liu Z. Proc Natl Acad Sci U S A. 2017 Dec 28. pii: 1713895115. doi: 10.1073/pnas.1713895115. 10.1073/pnas.1713895115 PubMed 29284749