pMXS-IRES-Blast SHMT2 CD
(Plasmid #106302)

• Purpose: Expresses SHMT2 K280A catalytic site mutant
• Depositing Lab: Richard Possemato
• Publication: Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5. doi: 10.1016/j.molcel.2018.01.024.

Backbone

• Vector backbone: pMXS-IRES-blast
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7000
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: serine hydroxymethyltransferase 2
• Alt name: SHMT2
• Species: H. sapiens (human)
• Insert Size (bp): 1518
• Mutation: K280A catalytic site mutation, Silent mutations destroy sgRNA targeting sites
• GenBank ID: NM_005412 NM_005412
• Entrez Gene: SHMT2 (a.k.a. GLYA, HEL-S-51e, NEDCASB, SHMT, mSHMT)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: AGTAGACGGCATCGCAGCTTGGATA
• 3′ sequencing primer: GGC GGA ATT TAC GTA GCG GCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry