pJL1 Aquamarine
(Plasmid #106285)

• Purpose: Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter
• Depositing Lab: James Collins
• Publication: Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. eCollection 2018 Aug.

Backbone

• Vector backbone: pJL1
• Backbone manufacturer: Michael Jewett Lab
• Backbone size w/o insert (bp): 2724
• Total vector size (bp): 2982
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Aquamarine
• Alt name: ECFP-T65S-H148G
• Species: A. victoria
• Insert Size (bp): 792
• Mutation: T65S and H148G
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis and TEV cleavage (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GAAATTAATACGACTCACTATAGGGAGACC
• 3′ sequencing primer: GCTCGAGGTTATCCGGATATAGTTC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Fabienne Merola (Addgene construct 42889)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry