pHAGE-GFP
(Plasmid #106281)

• Purpose: A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.
• Depositing Lab: Jay Shendure
• Publication: Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.4604. doi: 10.1038/nmeth.4604.

Backbone

• Vector backbone: pHAGE
• Total vector size (bp): 8442
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
• Species: Synthetic
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • phage_gfp_ires_dsred_wpre_mcs.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry