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Addgene

pTS36.6
(Plasmid #106264)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106264 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVG2.2
  • Backbone manufacturer
    Meyer et al. 2011 (PMID: 21378046)
  • Backbone size w/o insert (bp) 7814
  • Vector type
    Luciferase, Synthetic Biology
  • Selectable markers
    pyrG

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow for 2 days on agar
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    luc-P2A*-ekivR-P2A*-esyn1
  • Mutation
    P2A: changed final proline to alanine
  • Promoter Tet-On

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAAGTCGAGCTCCCCATC
  • 3′ sequencing primer GCTGACATCGACACCAAC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Grow for 2 days on agar

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTS36.6 was a gift from Vera Meyer (Addgene plasmid # 106264 ; http://n2t.net/addgene:106264 ; RRID:Addgene_106264)

  • For your References section:

    Polycistronic gene expression in Aspergillus niger. Schuetze T, Meyer V. Microb Cell Fact. 2017 Sep 25;16(1):162. doi: 10.1186/s12934-017-0780-z. 10.1186/s12934-017-0780-z [pii] PubMed 28946884
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