Skip to main content
Addgene

AAV-U6-dgRNA-CAG-MPH
(Plasmid #106259)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    AAV
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MS2-P65-HSF1
  • gRNA/shRNA sequence
    SapI
  • Species
    H. sapiens (human), Synthetic
  • GenBank ID
  • Entrez Gene
    HSF1 (a.k.a. HSTF1)
  • Promoter CAG

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene 61426

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MPH was constructed from plasmid lenti_MS2-P65-HSF1_Hygro (Addgene 61426)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-U6-dgRNA-CAG-MPH was a gift from Juan Belmonte (Addgene plasmid # 106259 ; http://n2t.net/addgene:106259 ; RRID:Addgene_106259)

  • For your References section:

    In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation. Liao HK, Hatanaka F, Araoka T, Reddy P, Wu MZ, Sui Y, Yamauchi T, Sakurai M, O'Keefe DD, Nunez-Delicado E, Guillen P, Campistol JM, Wu CJ, Lu LF, Esteban CR, Izpisua Belmonte JC. Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7. 10.1016/j.cell.2017.10.025 PubMed 29224783
Related items:
Commonly requested with: