AAV-U6-dgRNA-CAG-MPH
(Plasmid #106259)

• Purpose: Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone
• Depositing Lab: Juan Belmonte
• Publication: Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7.

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Backbone

• Vector backbone: AAV
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MS2-P65-HSF1
• gRNA/shRNA sequence: SapI
• Species: H. sapiens (human), Synthetic
• GenBank ID:
• Entrez Gene: HSF1 (a.k.a. HSTF1)
• Promoter: CAG

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • AAV-U6-dgRNA-CAG-MPH.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene 61426

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MPH was constructed from plasmid lenti_MS2-P65-HSF1_Hygro (Addgene 61426)