pcDNA5/FLAG-HA-DGCR8
(Plasmid #106251)

• Purpose: Express DGCR8 in mammalian cells
• Depositing Lab: Joshua Mendell
• Publication: Chang et al Genome Res. 2015 Sep;25(9):1401-9. doi: 10.1101/gr.193607.115.

Backbone

• Vector backbone: pcDNA5/FRT
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5070
• Total vector size (bp): 7524
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DiGeorge Syndrome Critical Region 8
• Alt name: DGCR8
• Species: H. sapiens (human)
• Insert Size (bp): 2442
• GenBank ID: NM_022720.6
• Entrez Gene: DGCR8 (a.k.a. C22orf12, DGCRK6, Gy1, pasha)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG-HA (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pFlag/HA-DGCR8 (Thomas Tuschl)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry