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Addgene

pLV hUbC-dSaCas9-KRAB-T2A-PuroR
(Plasmid #106249)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106249 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Backbone size w/o insert (bp) 10000
  • Total vector size (bp) 14000
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dead S. aureus Cas9 KRAB T2A PuroR
  • Alt name
    nuclease deactivated SauCas9
  • Species
    S. aureus
  • Mutation
    D10A and N580A
  • Promoter hUbC
  • Tags / Fusion Proteins
    • HA-NLS (N terminal on insert)
    • NLS-KRAB (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV hUbC-dSaCas9-KRAB-T2A-PuroR was a gift from Charles Gersbach (Addgene plasmid # 106249 ; http://n2t.net/addgene:106249 ; RRID:Addgene_106249)

  • For your References section:

    RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors. Thakore PI, Kwon JB, Nelson CE, Rouse DC, Gemberling MP, Oliver ML, Gersbach CA. Nat Commun. 2018 Apr 26;9(1):1674. doi: 10.1038/s41467-018-04048-4. 10.1038/s41467-018-04048-4 PubMed 29700298
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