Lyn-iTrkA-mCh-CRY2
(Plasmid
#106169)
-
Purposemammalian expression of Lyn-tagged intracellular domain of TrkA (aa 450-799) fused to mCherry and CRY2PHR (aa 1-498)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 106169 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepmCherry N1
- Backbone size w/o insert (bp) 3800
- Total vector size (bp) 7200
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameLyn iTrkA mCherry CRY2PHR
-
Alt nameCRY2
-
Alt nameTrkA
-
SpeciesR. norvegicus (rat), A. thaliana (mustard weed)
-
Entrez GeneCRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Lyn-iTrkA-mCh-CRY2 was a gift from Bianxiao Cui (Addgene plasmid # 106169 ; http://n2t.net/addgene:106169 ; RRID:Addgene_106169) -
For your References section:
Optical Activation of TrkA Signaling. Duan L, Hope JM, Guo S, Ong Q, Francois A, Kaplan L, Scherrer G, Cui B. ACS Synth Biol. 2018 Jul 20;7(7):1685-1693. doi: 10.1021/acssynbio.8b00126. Epub 2018 Jul 12. 10.1021/acssynbio.8b00126 PubMed 29975841