pCI syn iGluu
(Plasmid #106122)

• Purpose: Fast indicator for synaptic glutamate imaging (synapsin promoter)
• Depositing Lab: Katalin Torok
• Publication: Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 10.1073/pnas.1720648115.

Backbone

• Vector backbone: pCI
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 5526
• Modifications to backbone: CMV promoter replaced by synapsin promoter
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iGluSnFR S72T variant
• Alt name: iGluu
• Species: Escherichia coli (strain K12)
• Insert Size (bp): 1570
• Mutation: Changed Ser 72 to Thr
• GenBank ID: 21239023 21239023
• Promoter: synapsin
• Tags / Fusion Proteins:
  • PDGFR (C terminal on insert)
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7 forward

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCI syn iGluu is the S73T variant of pCI syn iGluSnFR.
(iGluSnFR was created by Marvin et al.2013)

bioRxiv: https://doi.org/10.1101/233494