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Addgene

pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant
(Plasmid #106112)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106112 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFRT_TO_FlagHA_dest
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PRKRA
  • Species
    H. sapiens (human)
  • Mutation
    silent mutation of Gly32 (GGG-->GGT)
  • Entrez Gene
    PRKRA (a.k.a. DYT16, HSD14, PACT, RAX)
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant was a gift from Thomas Tuschl (Addgene plasmid # 106112 ; http://n2t.net/addgene:106112 ; RRID:Addgene_106112)

  • For your References section:

    The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated Stress Response and Cell Cycle Progression. Meyer C, Garzia A, Mazzola M, Gerstberger S, Molina H, Tuschl T. Mol Cell. 2018 Feb 15;69(4):622-635.e6. doi: 10.1016/j.molcel.2018.01.011. 10.1016/j.molcel.2018.01.011 PubMed 29429924
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