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Addgene

Cerulean-2xFv-hRIPK3
(Plasmid #106079)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106079 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    FACS sort for Cerulean

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cerulean-2xFv-hRIPK3
  • Alt name
    oligomerizable human RIPK3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2950
  • GenBank ID
    NM_006871.3
  • Entrez Gene
    RIPK3 (a.k.a. RIP3)
  • Promoter LTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer AGATCTACCATGGTGAGCAAGGGCGAGGAG
  • 3′ sequencing primer GTCGACTTATTTCCCGCTATGATTATA
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cerulean-2xFv-hRIPK3 was a gift from Douglas Green (Addgene plasmid # 106079 ; http://n2t.net/addgene:106079 ; RRID:Addgene_106079)

  • For your References section:

    Sequential Engagement of Distinct MLKL Phosphatidylinositol-Binding Sites Executes Necroptosis. Quarato G, Guy CS, Grace CR, Llambi F, Nourse A, Rodriguez DA, Wakefield R, Frase S, Moldoveanu T, Green DR. Mol Cell. 2016 Feb 18;61(4):589-601. doi: 10.1016/j.molcel.2016.01.011. Epub 2016 Feb 4. 10.1016/j.molcel.2016.01.011 PubMed 26853145
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